منابع مشابه
Refactoring bacteriophage T7
Natural biological systems are selected by evolution to continue to exist and evolve. Evolution likely gives rise to complicated systems that are difficult to understand and manipulate. Here, we redesign the genome of a natural biological system, bacteriophage T7, in order to specify an engineered surrogate that, if viable, would be easier to study and extend. Our initial design goals were to p...
متن کاملFlap endonuclease of bacteriophage T7
Gene 6 protein of bacteriophage T7 has 5'-3'-exonuclease activity specific for duplex DNA. We have found that gene 6 protein also has flap endonuclease activity. The flap endonuclease activity is considerably weaker than the exonuclease activity. Unlike the human homolog of gene 6 protein, the flap endonuclease activity of gene 6 protein is dependent on the length of the 5'-flap. This dependenc...
متن کاملSuperinfection exclusion by bacteriophage T7.
Only two of the early genes of bacteriophage T7 were found to play a significant role in exclusion of superinfecting bacteriophage T3 particles; genes 0.3 and 1. Protein synthesis by the preinfecting phage particle was not required for efficient exclusion. These findings are discussed with regard to the known functions of these genes during T7 development.
متن کاملBacteriophage T7 DNA polymerase – sequenase
An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high fidelity in the absence of proofreading/exonuclease activity; and (4) production of clear and uniform signals for detection. The DNA polymerase...
متن کاملOrder of injection of T7 bacteriophage DNA.
The deoxyribonucleic acid (DNA) corresponding to the left-hand end of the genetic map is injected first in T7 phage infection. The order was deduced by use of marker rescue experiments to see which genes are still injected after the double-stranded DNA chain is broken by X rays. The treatment causes a selective loss of genes located on the right side of the map. Presumably, this corresponds to ...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1968
ISSN: 0003-6919
DOI: 10.1128/aem.16.10.1483-1488.1968